Objective To investigate the effects of hammock positioning on the growth and development indexes of premature infants,such as weight,body length and head circumference. Methods From January to August 2020,106 premature infants admitted to a tertiary children’s hospital in Hangzhou were selected as the research subjects. They were divided into an experimental group(n=53) and a control group(n=53) by random number table method. The control group was treated with conventional heating box. The experimental group was treated with hammock positioning in addition to conventional warm box care. The growth and development of infants in the 2 groups were compared. Results The weight gain of premature infants in the experimental group at the 2nd to 4th weeks,the 24 - hour sleep duration and the discharge weight were higher than those of the control group;the number of days required to restore birth weight and the length of hospital stay are lower than those of the control group,and the difference was statistically significant(P<0.05). After using hammock therapy,the heart rate and respiratory rate of the experimental group decreased,and the oxygen saturation increased,and the difference was statistically significant(P<0.05). Conclusion Hammock positioning plays a positive role in promoting the average weight gain per week,reducing the number of days to regain birth weight,increasing the duration of 24 hours sleep,stabilizing vital signs of premature infants and reducing the length of hospital stay,which can effectively promote the growth and development of premature infants.